السيناريو
A researcher is performing western blot to detect a target protein from cell lysate. The protocol involves steps from sample preparation to imaging, with a decision on primary antibody incubation duration.
ما الذي يتضمنه هذا الرسم
اقرأ القرارات الكامنة وراءه.
01Choose primary antibody incubation
overnight at 4°C or 2 hours at RT.
For training new lab members, writing methods sections, or planning western blot experiments.