The scenario
A researcher is performing western blot to detect a target protein from cell lysate. The protocol involves steps from sample preparation to imaging, with a decision on primary antibody incubation duration.
What is in this drawing
Read the decisions behind it.
01Choose primary antibody incubation
overnight at 4°C or 2 hours at RT.
For training new lab members, writing methods sections, or planning western blot experiments.
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