El escenario
A microbiology lab performs the Schaeffer-Fulton method to differentiate Bacillus subtilis endospores from vegetative cells based on differential staining.
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01
Use steam heat to drive malachite green into the endospore coat
02
Rinse with water to decolorize vegetative cells while retaining stain in spores
03
Counterstain with safranin to color vegetative cells pink
Use this protocol when teaching or performing endospore staining to visualize and confirm spore-forming bacteria such as Bacillus subtilis.