MICROBIOLOGY
Schaeffer-Fulton Endospore Staining Procedure for Bacillus subtilis
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CASE ANALYSIS
Scenario
A microbiology lab performs the Schaeffer-Fulton method to differentiate Bacillus subtilis endospores from vegetative cells based on differential staining.
Key decisions
- Use steam heat to drive malachite green into the endospore coat
- Rinse with water to decolorize vegetative cells while retaining stain in spores
- Counterstain with safranin to color vegetative cells pink
When to reuse this
Use this protocol when teaching or performing endospore staining to visualize and confirm spore-forming bacteria such as Bacillus subtilis.
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