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Microbiology · Laboratory Protocol · Bacterial Identification

Gram Stain Flowchart for Differentiating Bacterial Cultures

Type FlowchartStandard Sugiyama layered DAG + orthogonal routingEngine schematex-flowchartUpdated 9/15/2026
Gram Stain Flowchart for Differentiating Bacterial Cultures
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The scenario

A laboratory technician follows a standardized Gram staining procedure to classify an unknown bacterial isolate based on cell wall properties and microscopic morphology.

What is in this drawing

Read the decisions behind it.

01

Purple vs pink/red color after staining

02

Cocci clusters vs chains vs bacilli for Gram-positive organisms

03

Cocci vs bacilli vs coccobacilli for Gram-negative organisms

Use when teaching or executing the Gram stain protocol to presumptively identify bacterial groups from a pure culture.

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