MICROBIOLOGY
India Ink Negative Staining Protocol for Cryptococcus Detection
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CASE ANALYSIS
Scenario
Clinical microbiology laboratory performing India Ink staining on cerebrospinal fluid to detect encapsulated yeast.
Key decisions
- India ink vial within expiry
- Preparation adequate and capsular halos visible
- Yeast-like cells present without capsules
When to reuse this
Use when training or documenting the negative staining workflow for Cryptococcus neoformans capsule detection.
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flowchart-india-ink-negative-staining-for-cryptococcus-neoformans-caps